Acoustic piezoelectric resonators are widely used as precise analytical chemistry tools for the real-time monitoring of a negligibly small amount of surface-attached mass of biological components, in particular, in environmental biosensor measurements. The surface acoustic wave (SAW)-based sensors and the quartz crystal microbalance (QCM) compared in our work belong to the leading group due to their considerable advantages. These piezoelectric resonators are considered now as high-resolution analytical tools allowing researchers to discriminate between components due to the selective polymer coating on the resonator surface. The gravimetrical measurements performed with the SAW-based or QCM sensors provide the experimental data with high precision for the detection of surface mass for the thin adsorbed layer rigidly attached to the oscillator surface. The new challenge is the analysis of soft and biological materials, where the viscous losses of energy can essentially influence measured characteristics. Modelling is the important part of the analysis allowing researchers to quantify the results of the experiments. The present work provides a general theory of SH-SAW devices probing soft and biological materials. The results are compared with QCM-D operated in liquid media.
Two types of devices are accepted as acoustical sensors now: SAW (surface acoustic wave)-based and BAW (bulk acoustic wave)-based resonators. The quartz crystal microbalance (QCM) sensors (BAW resonators), originally used for the gravimetric measurements in gases mostly (Sauerbrey, 1959), and later in viscous Newtonian liquids (Kanazawa and Gordon, 1985; Kanazawa et al., 2008; Rodahl and Kasemo, 1996; Rodahl et al., 1995, 1997), are widely used now for viscous/viscoelastic films studies (Johannsmann et al., 1992; Johannsmann, 2007, 2015; Lucklum and Hauptmann, 2000, 2006), as well as for biosensor purposes (Janshoff and Steinem, 2001; Marx, 2003; Rodahl et al., 1997). Surface acoustic wave sensors have a number of advantages in comparison with the QCM. First of all, it is the high resonance frequency (100 MHz – a few GHz frequencies operation region, while typically, 3–10 MHz one for the QCM). Surface acoustic wave resonators are commonly used in the air and gases (Gaso et al., 2013; Kalantar-Zadeh et al., 2001, 2003a, b, 2006; Kovacs and Venema, 1992; Länge et al., 2008; Penza et al., 1998). Starting from the original research in Love wave devices (Love, 1911) rapid development in SAW gas sensors was made due to appearance of new advanced materials for the sensors' surface coating (Penza et al., 2004, 2007, 2009). In spite of the experimental problems in the late 1980s and early 1990s, in a number of original works (Gizeli et al., 1992a, b, 1996, 1997) it was shown that SAW devices with horizontal wave polarization (SH SAWs) can be used for measurements in liquids, in particular, as viscosity sensors (Ricco and Martin, 1987; Kalantar-Zadeh et al., 2003; Powell et al., 2004). This finding opens a way for the biosensor applications since water is an essential component for the proteins, nucleic acids and other biological molecules. The specific chemical treatment of the resonator surface permits the highly selective detection of biological components in a water solution, enabling the SH-SAW devices for the in situ monitoring of protein adsorption, bacteria toxins, DNA studies and immunological analysis (Freudenberg et al., 2001; Kalantar-Zadeh et al., 2003a, b; Länge et al., 2008; Rapp et al., 2007). Another engineering trend for the life science applications is a combination of SAW sensors with the peripheral microfluidics which is an excellent perspective, e.g. for the lab-on-a-chip and immunological setup construction (Länge et al., 2008; Melzak et al., 2007). Both methods – the QCM and SH-SAW resonators – are modern precise bioanalytical tools and both of them demonstrate beneficial features. The dynamics of a film immersed into a bulk Newtonian liquid and firmly attached to the oscillating crystal substrate can be studied with the help of acoustic resonators by using several piezomaterials with different acoustical characteristics. For the quartz, different cut of the crystal allows the excitation of either surface acoustic waves or bulk acoustic waves. For example, the AT-cut quartz plate is typically used for the quartz crystal microbalance resonators.
The theoretical modelling is important for the quantitative analysis and interpretation of the experimental results. Many efforts have been reported in this area since the 1990s (Gulyaev and Plessky, 1997; Gulyaev, 1998; Kalantar-Zadeh et al., 2003a, b; Kosevich et al., 1995; Newton et al., 2001; Powell et al., 2004; Kovacs and Venema, 1992; McHale et al., 2001; Plessky, 1995). The challenge is to include the softness (or viscoelasticity) of the testing material into consideration, specifically, when the resonator operates in a liquid. To our knowledge, this problem has not been solved analytically.
In the present paper, the viscoelastic correction was found for the characteristics of surface acoustic waves with horizontal polarization. The rigorous mathematical expressions for the shift in the phase wave velocity and attenuation (the dissipation coefficient) for this type of SAWs are derived for the first time. The results of the theory are compared with the “missing mass” effect predicted earlier (Voinova et al., 1999, 2002) for the BAWs (specifically important for QCM-D, a device which allows researchers the simultaneous measurements of the resonance frequency and the dissipation in liquid applications; Rodahl and Kasemo, 1996; Rodahl et al., 1995, 1997).
We study surface acoustic waves with horizontal polarization (SH waves)
propagating in the two-layer system. In the paper we employed our method
developed earlier in Kosevich et al. (1995) and Voinova et al. (1997) and
generalized the theory for the case of a viscoelastic thin layer under a bulk
liquid (water solution, for example). Such a system has been analysed in
details in our earlier works (Voinova et al., 1999, 2002; Voinova, 2009) for the BAWs
excited in the quartz crystal microbalance (QCM). In the last section of the
paper we compare the QCM results and our analytical results obtained for the
surface acoustic waves. The importance of this research is the analytical
formulae obtained for the shift in the phase velocity and the attenuation of
acoustic SH waves which can be measured experimentally and, thus, verified for
the coatings with known properties (for example, supported membranes or
polymer films). In the air, the surface acoustic wave phase velocity change
due to the presence of rigid two-layer film of different densities is given
by the following expression (Voinova et al., 1997):
The dependence of the phase velocity on the film surface mass makes it
possible to use Love wave devices for the gravimetrical measurements. For
bulk acoustic waves, the measured shift in the resonance frequency is
proportional to the surface mass and the frequency. The linear relationship
between the shift in the resonance frequency and the surface mass which
causes this shift (the principle of microbalance for the QCM devices) is
given by the so-called Sauerbrey relation (Sauerbrey, 1959):
In the next section the theoretical formulation of the model is presented for the SH-SAW propagated into a layered system onto the surface of a solid substrate. The similar physical phenomenon of the “missing mass” found in the calculated shift of the phase velocity of the resonator when the upper layer is a viscous bulk liquid.
We analyse the surface acoustic waves with horizontal polarization
propagating in the two-layer system with the geometry depicted on Fig. 1. The
viscoelastic properties of the surface-adjacent thin film are described with
the complex shear modulus of elasticity
Sketch of the two-layer system deposited onto the surface of SH-surface acoustic resonator: the first layer is a supported membrane; the second is the bulk aqueous solution.
The displacement of the solid substrate boundary is given by
At
Equations (1) and (2) together with the boundary conditions (3)–(6) lead to the dispersion equation which is presented and analysed in the next section.
The dispersion equation in the long-wave approximation is given by the
expression
From the analysis of the dispersion relation (7) in the long wavelength
approximation, for the acoustically thin film and for
In the contrast, for the pure elastic thin film considering “dry
conditions” (for example, when the resonator operated in gaseous
environment) it was found (Kosevich et al., 1995) that the phase velocity
shift is proportional to the square of the surface film mass and frequency:
To compare the results derived here for the surface acoustic waves with the
horizontal polarization, we also present the analytical expressions for the
characteristics of QCM-D, namely, for the resonance frequency shift,
The expressions (8) and (9) presented in this work reveal the “missing mass” effect for the SH-SAW case, i.e. show the necessity of the correction of surface mass deduced from SAW device measurements in liquids.
Considering the results (Eqs. 8, 9) of the present paper, one can deduce that, in the case of surface acoustic wave propagation in two-layer geometry when the bulk layer is a viscous Newtonian liquid, calculation has shown that the phase velocity is proportional to the first order to the surface mass while in the air only to the square value (we would like to point out here that the calculations were performed in the approximation when the film thickness is taken as a small parameter).
Formulae (8) and (9), reporting the “viscous correction” of characteristics in the present paper, can provide valuable theoretical support for the liquid phase measurements using the surface acoustic wave sensors. The detailed analysis of the “missing mass” effect will be given in the second part of this work (Voinova and Vikström, 2015).
The author thanks Chalmers University of Technology for continuing support. Edited by: Y. Li Reviewed by: two anonymous referees