Development and characterisation of a new fluorescence sensor for online monitoring of bioprocesses
Jan C. König,Tobias Steinwedel,Dörte Solle,Patrick Lindner,Ingo de Vries,Thorleif Hentrop,Michael Findeis,Gernot T. John,Thomas Scheper,and Sascha Beutel
Jan C. König
Institute of Technical Chemistry, Leibniz University of Hanover,
30167 Hanover, Germany
Tobias Steinwedel
Institute of Technical Chemistry, Leibniz University of Hanover,
30167 Hanover, Germany
Dörte Solle
Institute of Technical Chemistry, Leibniz University of Hanover,
30167 Hanover, Germany
Patrick Lindner
Institute of Technical Chemistry, Leibniz University of Hanover,
30167 Hanover, Germany
Ingo de Vries
Institute of Technical Chemistry, Leibniz University of Hanover,
30167 Hanover, Germany
Thorleif Hentrop
Institute of Technical Chemistry, Leibniz University of Hanover,
30167 Hanover, Germany
Michael Findeis
PreSenS GmbH, 93053 Regensburg, Germany
Gernot T. John
PreSenS GmbH, 93053 Regensburg, Germany
Thomas Scheper
Institute of Technical Chemistry, Leibniz University of Hanover,
30167 Hanover, Germany
Fluorescence spectroscopy represents a very sensitive method, but fluorescence sensors are mainly used in academia and rarely in industrial processes. In this work, we present a newly developed functional model of an online fluorescence sensor. The sensor was miniaturised and constructed without any movable parts to detect important biologic fluorophores. Its performance was evaluated by calibration curves and selectivity tests and it was used for a biomass prediction of E. coli cultivations.
Fluorescence spectroscopy represents a very sensitive method, but fluorescence sensors are...