Articles | Volume 7, issue 2
https://doi.org/10.5194/jsss-7-461-2018
https://doi.org/10.5194/jsss-7-461-2018
Regular research article
 | 
08 Aug 2018
Regular research article |  | 08 Aug 2018

Development and characterisation of a new fluorescence sensor for online monitoring of bioprocesses

Jan C. König, Tobias Steinwedel, Dörte Solle, Patrick Lindner, Ingo de Vries, Thorleif Hentrop, Michael Findeis, Gernot T. John, Thomas Scheper, and Sascha Beutel

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Cited articles

Assawajaruwan, S., Eckard, P., and Hitzmann, B.: On-line monitoring of relevant fluorophores of yeast cultivations due to glucose addition during the diauxic growth, Process Biochem., 58, 51–59, https://doi.org/10.1016/j.procbio.2017.05.007, 2017a. 
Assawajaruwan, S., Reinalter, J., and Hitzmann, B.: Comparison of methods for wavelength combination selection from multi-wavelength fluorescence spectra for on-line monitoring of yeast cultivations, Anal. Bioanal. Chem., 409, 707–717, https://doi.org/10.1007/s00216-016-9823-2, 2017b. 
Boehl, D., Solle, D., Hitzmann, B., and Scheper, T.: Chemometric modelling with two-dimensional fluorescence data for Claviceps purpurea bioprocess characterization, J. Biotechnol., 105, 179–188, 2003. 
Claßen, J., Aupert, F., Reardon, K. F., Solle, D., and Scheper, T.: Spectroscopic sensors for in-line bioprocess monitoring in research and pharmaceutical industrial application, Anal. Bioanal. Chem., 409, 651–666, https://doi.org/10.1007/s00216-016-0068-x, 2017. 
Clavaud, M., Roggo, Y., von Daeniken, R., Liebler, A., and Schwabe, J.-O.: Chemometrics and in-line near infrared spectroscopic monitoring of a biopharmaceutical Chinese hamster ovary cell culture: prediction of multiple cultivation variables, Talanta, 111, 28–38, https://doi.org/10.1016/j.talanta.2013.03.044, 2013. 
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Short summary
Fluorescence spectroscopy represents a very sensitive method, but fluorescence sensors are mainly used in academia and rarely in industrial processes. In this work, we present a newly developed functional model of an online fluorescence sensor. The sensor was miniaturised and constructed without any movable parts to detect important biologic fluorophores. Its performance was evaluated by calibration curves and selectivity tests and it was used for a biomass prediction of E. coli cultivations.