Articles | Volume 7, issue 2
https://doi.org/10.5194/jsss-7-433-2018
https://doi.org/10.5194/jsss-7-433-2018
Regular research article
 | 
06 Jul 2018
Regular research article |  | 06 Jul 2018

Metal ion binding and tolerance of bacteria cells in view of sensor applications

Jonas Jung, Anja Blüher, Mathias Lakatos, and Gianaurelio Cuniberti

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Cited articles

Blüher, A., Ostermann, K., Jäckel, P., Clemens, A., Katzschner, B., Rödel, G., and Mertig, M.: Extraction and long-term storage of S-layer proteins and flagella from Lysinibacillus sphaericus NCTC 9602: Building blocks for nanotechnology, Eng. Life Sci., 15, 410–415, https://doi.org/10.1002/elsc.201400182, 2015. 
Conroy, D. J., Millner, P. A., Stewart, D. I., and Pollmann, K.: Biosensing for the environment and defence: Aqueous uranyl detection using bacterial surface layer proteins, Sensors, 10, 4739–4755, 2010. 
Fahmy, K., Merroun, M., Pollmann, K., Raff, J., Savchuk, O., Hennig, C., and Selenska-Pobell, S.: Secondary structure and Pd (II) coordination in S-layer proteins from Bacillus sphaericus studied by infrared and X-ray absorption spectroscopy, Biophys. J., 91, 996–1007, 2006. 
Fano, U.: The theory of anomalous diffraction gratings and of quasi-stationary waves on metallic surfaces (Sommerfeld's waves), J. Opt. Soc. Am., 31, 213–222, 1941. 
Geoghegan, W. D. and Ackerman, G. A.: Adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat anti-human immunoglobulin G on cell surfaces at the electron microscopic level: a new method, theory and application, J. Histochem. Cytochem., 25, 1187–1200, 1977. 
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Short summary
We assessed the applicability of bacterial surface layer proteins of Lysinibacillus sphaericus JG-B53 and Sporosarcina ureae ATCC 13881 for the detection of metal ions in water. Based on the interactions of the cell components with metal complexes, two potential sensor systems, one colorimetric with functionalized gold nanoparticles and the other using a regenerative sensor layer, were developed. The systems' detection limits of YCl3 in water were 1.67 x 10−5 and 1 x 10−4 mol L−1, respectively.