Articles | Volume 6, issue 2
https://doi.org/10.5194/jsss-6-351-2017
https://doi.org/10.5194/jsss-6-351-2017
Regular research article
 | 
16 Oct 2017
Regular research article |  | 16 Oct 2017

Transmitted light pH optode for small sample volumes

Christian Rogge, Steffen Zinn, Paolo Prosposito, Roberto Francini, and Andreas H. Foitzik

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Cited articles

Brown, J. D., Bell, N., Li, V., and Cantrell, K.: Quantitative pH assessment of small-volume samples using a universal pH indicator, Anal. Biochem., 462, 29–31, 2014.
Capel-Cuevas, S., Cuéllar, M., de Orbe-Payá, I., Pegalajar, M., and Capitán-Vallvey, L.: Full-range optical pH sensor based on imaging techniques, Anal. Chim. Acta, 681, 71–81, 2010.
Chen, H.-X., Wang, X.-D., Song, X.H., Zhou, T.-Y., Jiang, Y.-Q., and Chen, X.: Colorimetric optical pH sensor production using a dual-color system, Sensor. Actuat. B-Chem., 146, 278–282, 2010.
Dean, J.: Lange's Handbook of Chemistry, McGraw-Hill, 14th Edn., chap. 8, 8.115–8.116, 1992.
Di Veroli, G. Y., Fornari, C., Goldlust, I., Mills, G., Koh, S. B., Bramhall, J. L., Richards, F. M., and Jodrell, D. I.: An automated fitting procedure and software for dose-response curves with multiphasic features, Sci. Rep.-UK, 5, 14701, https://doi.org/10.1038/srep14701, 2015.
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Short summary
This work presents a cost-effective optical-based sensor for pH monitoring of sample volumes less than 150 µL. As part of a bigger project to develop a stand-alone micro bioreactor system, measurements carried out with phenol red and DMEM reported a standard error of calibration in the physiologic pH range (6.5–7.5) of pH ±0.04. Compared to other accessible optical-based sensors this reported system is a good alternative regarding the overall costs of less than EUR 50.